Endodontic State of Art (Dr Kolnick)

 - Review of Literature for Er,Cr:YSGG lasers in Endodontics

Total elimination of bacteriafrom infected root-canal systems remains the most important objective of endodontic therapy. However, in spite of a plethora of new products and techniques, achieving this objective continues to elude our profession. Historically, endodontic treatment focused on root canal disinfection with “entombment” of remainingbacteria within dentinal tubules and inaccessible areas of the root-canal system.

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Combined Sodium Hypochlorite and 940 nm Diode Laser Treatment Against Mature E. Faecalis Biofilms in-vitro

Abstract:

Background: Laser irradiation has been introduced in endodontic treatment due to its bactericidal effect. The aim of this study is to evaluate the bactericidal efficacy of a 940 nm diode laser alone or in combination with 5% sodium hypochlorite (NaOCl) against mature biofilms of E. Faecalis.

Methods: Sixty-eight (60 for the three groups, 4 for SEM and 4 as negative controls) single-rooted human central incisors were prepared and contaminated with E. Faecalis. After two weeks of incubation, specimens were randomly divided in three groups; group 1 (n =20), the teeth were irradiated with a 940 nm diode laser; group 2 (n=20), specimens were rinsed with 5% NaOCl; group 3 (n=20), the teeth were rinsed with 5% NaOCl and then were irradiated with 940 nm diode laser. Four teeth were used to observe the biofilms by SEM. Intracanal bacteria sampling was done, and the samples were plated to determinate the CFU count.

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